World Grain - November 2019 - 52

FEATURE: ALLERGEN AND MYCOTOXIN DETECTION

Grain facilities
can detect and
quantify five
major mycotoxins
using a single,
solvent-free
sample extraction
through VICAM's
recently launched
Myco 5-in-1
lateral flow strip
test solution.
Photo courtesy
of Vicam.

ORZLQJIHHGPLOOVWRSURFHVVWUXFNVDWDIDVWHUUDWH7KHVH
quantitative mycotoxin tests are inserted into an instruPHQWWKDWSURFHVVHVWHVWUHVXOWVDQGPDNHVWKHPDYDLODEOH
for digital distribution."
"A growing challenge for grain operations is the need to
screen and quantify more than one mycotoxin in a single
LQERXQG RU RXWERXQG VKLSPHQW´ VDLG 3DWULFLD -DFNVRQ
PDUNHWGHYHORSPHQWPDQDJHU9,&$0³*OREDOUHJXODWRU\ OLPLWV DQG HQGXVHU TXDOLW\ VSHFL¿FDWLRQV VXFK DV
corn millers, biofuels, feed or pet food manufacturers) often require grains to meet strict maximum contamination
OHYHOVIRUDÀDWR[LQIXPRQLVLQYRPLWR[LQ'21]HDUDlenone and ochratoxin prior to acceptance."
MYCOTOXIN TEST METHODS           
Mycotoxin distribution in grains is largely non-homogeneous since often only a reduced amount of materials in
EXONLVFRQWDPLQDWHGDWYHU\KLJKOHYHOV7KHUHIRUHLQWKH
HYDOXDWLRQRIWKHOHYHORIP\FRWR[LQLQEXONWKHHUURUDVsociated with the sampling step is the most relevant contribution to the overall uncertainty. Accordingly, to obtain
DODERUDWRU\UHVXOWWKDWUHÀHFWVWKHOHYHORIWR[LQSUHVHQWLQ
a lot of product, standard sampling and laboratory preparation procedures should be followed.
 %,20,1 FODVVL¿HV P\FRWR[LQ DQDO\VLV PHWKRGV DV
reference testing methods: Thin Layer Chromatography
(TLC); Gas Chromatography (GC); High-Performance
Liquid Chromatography (HPLC) and Liquid
Chromatography-Mass Spectrometry (LC/MS); and rapLG WHVWLQJ PHWKRGV /DWHUDO )ORZ 7HVW (Q]\PH/LQNHG
,PPXQRVRUEHQW$VVD\(/,6$DQG)OXRURPHWU\
x TLC: The TLC technique represents the most economical method in mycotoxin analysis and is widely used in developing countries. However, its sensiWLYLW\LVOLPLWHGLWGRHVQRWZRUNIRUDOOP\FRWR[LQV
and it uses solvents that regard ecological hazard.
New detection techniques for TLC have been de-

52

veloped as alternatives to traditional TLC scanners.
x GC: Gas chromatography-based methods represent the most widely employed techniques
for the determination of trichothecenes, as deoxynivalenol and nivalenol, since these compounds do not intensely absorb in the UV-visLEOH UDQJH DQG DUH QRQÀXRUHVFHQW VR WKDW WKH
use of mass spectrometry (GC/MS) is in some
FDVHVFUXFLDOLQWKHLGHQWL¿FDWLRQVWHS
x HPLC: A wide variety of methods for most
mycotoxins are based on HPLC. The advantages of HPLC methods include excellent
performance characteristics, low detection
level, and safety for the operator. However, it
is time-consuming, expensive and requires a
trained technician.
x LC-MS: The LC-MS technique is relatively
popular, mainly due to well-established, sensiWLYH DQG UHOLDEOH /& WHFKQLTXHV ZLWK ÀXRUHVcence detectors with the ability to measure the
QDWXUDO ÀXRUHVFHQFH RI WR[LQV 3HUNLQ(OPHU¶V
QSight Triple Quad 400 Series (a LC/MS/
06 V\VWHP SURYLGHV DGYDQFHG FRQ¿UPDWLRQ
testing and analyzes multiple types of mycoWR[LQV ² OLNH DÀDWR[LQ YRPLWR[LQ ]HDUDOHnone, fumonisin, T-2 and Ochratoxin A - in
grain products such as wheat, corn, soy and
more at large processors and contract labs,
said MacBeath. Amin Mousavi Khaneghah et
al., 2019, reviewed published studies on mycotoxins in cereal-based products for 24 years
(1983-2017) and reported that the lowest and
highest number of published reports was assoFLDWHGZLWK'21DQGWRWDODÀDWR[LQLQVRPHRI
the cereal-based products, respectively. They
also found that the liquid chromatography-electrospray ionization-tandem mass spectrometry
November 2019 / World Grain / www.World-Grain.com


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World Grain - November 2019

Table of Contents for the Digital Edition of World Grain - November 2019

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